Growth conditions:
-
Axenic Lemna gibba in E-medium
+ 1% sucrose,
-
temperature 25 C,
-
continuous light 40-55 µEinstein•m-2•sec-2
from four fluorescent tubes, 2xF40cw and 2xF40Gro.
Definitions:
Photosynthetically active radiation (PAR) is generally measured
in units of µEinsteins. A µEinstein of light is Avogadro's
number (6.02 x 1023 = 1 mole) of photons.
Full sunlight is approximately 2000 µEinsteins•m-2•sec-2.
The intensity of PAR in this experiment, 50 µEinsteins•m-2•sec-2,
is near the compensation
point for photosynthesis, the light intensity for which photosynthesis
just balances respiration. For that reason, axenic culture using
sucrose was necessary to obtain good growth.
-
For more information about photosynthesis, see [ this
summary ] from a course at the University of Illinois, Urbana/Champaign.
The same group also recommend these [ links
].