Axenic Lemna gibba in E-medium
+ 1% sucrose,
temperature 25 C,
continuous light 40-55 µEinstein•m-2•sec-2
from four fluorescent tubes, 2xF40cw and 2xF40Gro.
Photosynthetically active radiation (PAR) is generally measured
in units of µEinsteins. A µEinstein of light is Avogadro's
number (6.02 x 1023 = 1 mole) of photons.
Full sunlight is approximately 2000 µEinsteins•m-2•sec-2.
The intensity of PAR in this experiment, 50 µEinsteins•m-2•sec-2,
is near the compensation
point for photosynthesis, the light intensity for which photosynthesis
just balances respiration. For that reason, axenic culture using
sucrose was necessary to obtain good growth.
For more information about photosynthesis, see [ this
summary ] from a course at the University of Illinois, Urbana/Champaign.
The same group also recommend these [ links
Copyright (c) 1999 John W. Cross
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